5447 – Bulk HIV-1 p24 10 plates/kit

SKU 5447 Categories ,

$5,295.00

This product is for research purposes only

Description

HIV-1 p24 ELISA Kits| 10 Assay kit (96-well plates)

The ABL p24 ELISA kit features a cost-effective & convenient protocol for quantification of HIV-1 & related lentiviral vector (LVV) titers.

Sensitive

    • Quantitation down to picogram level: 3 to 100 pg/mL.

Convenient

    • Pre-formulated reagents
    • 3 incubation steps
    • Removable 8-well strips

Efficient

    • Low sample volume: typically less than 10 µL
    • Results in under 3 hours

Robust

    • Strong correlation with genomic titer measurement
    • Results are stable over time compared to genomic titer analysis of stored RNA

Components

    • Microelisa Plate: 96-well plate coated with murine monoclonal antibodies to HIV-1 p24.
    • Conjugate Solution: Contains horseradish peroxidase-labeled, immunoaffinity purified, human antibodies to HIV-1 p24.
    • Peroxidase Substrate: Contains buffered hydrogen peroxide and TMB.
    • Purified native HIV-1 p24 Standard: 1 ng/mL.
    • Disruption Buffer: Contains Triton® X-100 detergent and phosphate buffer.
    • Wash Buffer (20X): Contains PBS / Tween 20® concentrate.
    • Stop Solution: Contains 2N sulfuric acid.

Components not sold separately.

References

Kuroishi, A., Saito, A., Shigai, Y., Shioda, T., Nomaguchi, M., Adachi, A., Akari, H., Nakayama, E. Modification of a Loop Sequence between Alpha Helices 6 and 7 of Virus Capsid (CA) Protein in a Human Immunodeficiency Virus Type I (HIV-1) Derivative that has Simian Immunodeficiency  Virus (SIVmac239) vif and CA Alpha-Helices 4 and 5 Loop Improves Replication in Cynomolgus Monkey Cells.   Retrovirology, 6:70.  2009.

Humeau, L., Binder, G., Lu, X., Slepushkin, V., Merling, R., Echeagaray, P., Pereira, M., Slepushkina, T., Barnett, S., Dropulic, L., Carroll, R., Levine, B., June, C., Dropulic, B.  Efficient Lentiviral Vector-Mediated Control of HIV-1 Replication in CD4 Lymphocytes from Diverse HIV Infected Patients Grouped According to CD4 Count and Viral Load.  Molecular Therapy 9(6): 902-913. 2004.

Supporting Documentation

Product Manual:

HIV-1 p24 ELISA Kit Manual

White Paper:
Performance Evaluation of the ABL p24 ELISA Kit for Accurate Lentiviral Vector Titer Determination in Cell and Gene Therapy Applications

Safety Data Sheets (SDS):

Disruption Buffer SDS 101315
Stop Solution SDS US 102015
HIV-1 p24 Antigen Capture Assay SDS US 101315

Certificates of Analysis:

Certificate of Analysis: Lot 2150
Certificate of Analysis: Lot 2151
Certificate of Analysis: Lot 2152
Certificate of Analysis: Lot 2153
Certificate of Analysis: Lot 2154
Certificate of Analysis: Lot 2155B
Certificate of Analysis: Lot 2156B
Certificate of Analysis: Lot 2157
Certificate of Analysis: Lot 2157C
Certificate of Analysis: Lot 2158
Certificate of Analysis: Lot 2159
Certificate of Analysis: Lot 2160C
Certificate of Analysis: Lot 2161B
Certificate of Analysis: Lot 2161C
Certificate of Analysis: Lot 2162
Certificate of Analysis: Lot 2161D
Certificate of Analysis: Lot 2163
Certificate of Analysis: Lot 2164
Certificate of Analysis: Lot 2165
Certificate of Analysis: Lot 2167
Certificate of Analysis: Lot 2168
Certificate of Analysis: Lot 2169
Certificate of Analysis: Lot 2170
Certificate of Analysis: Lot 2171
Certificate of Analysis: Lot 2172
Certificate of Analysis: Lot 2173
Certificate of Analysis: Lot 2174
Certificate of Analysis: Lot 2175
Certificate of Analysis: Lot 2176
Certificate of Analysis: Lot 2177
Certificate of Analysis: Lot 2178
Certificate of Analysis: Lot 2179

Contact Us

Do not hesitate to contact us should you have any questions or enquiries.
  • Please leave your comments